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Hydrolysis on the imine derives N-carbamoylputrescine followed with hydrolysis in the urea to kind putrescine. The independent pathways of converting ornithine to putrescine in plants and animals have converged. A SAM-dependent N-methylation of putrescine gives the N-methylputrescine products, which then undergoes oxidative deamination via the motion of diamine oxidase to produce the aminoaldehyde. Schiff foundation development confirms the biosynthesis on the N-methyl-Δ1-pyrrolinium cation.

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